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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
25/09/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, P. M. V.; GASPAR, C. G.; BUSS, D. S.; VENTURA, J. A.; FERREIRA, P. C. G.; FERNANDES, P. M. B. |
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Afiliação: |
Paolla M. V. Abreu, Universidade Federal do Espíríto Santo; Clicia G. Gaspar, Univervidade Federal do Rio de Janeiro; David S. Buss, Universidade Federal do Espírito Santo; Jose Aires Ventura, Incaper; Paulo C. G. Ferreira, Universidade Federal do Rio de Janeiro; Patricia M. B. Fernandes, Universidade Federal do Espírito Santo. |
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Título: |
Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
PLOS ONE, v. 9, issue 7, p. 1-13, july 2014. |
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Páginas: |
13 p. |
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Descrição Física: |
il., color. |
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ISSN: |
1932-6203 |
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DOI: |
10.1371 |
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Idioma: |
Português |
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Conteúdo: |
MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. MenosMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for thi... Mostrar Tudo |
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Palavras-Chave: |
Carica papaya; Doenças; Mamão; Meleira; MiRNAs; PMeV; Vírus. |
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Thesaurus NAL: |
Carica papaya; Disease; Meleira; MiRNAs; PMeV; Virus. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/397/1/Carica-papaya-microRNAS-Are-responsive-to-papaya.pdf
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Marc: |
LEADER 02852naa a2200373 a 4500
001 1004325
005 2015-09-01
008 2014 bl uuuu u00u1 u #d
022 $a1932-6203
024 7 $a10.1371$2DOI
100 1 $aABREU, P. M. V.
245 $aCarica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection.$h[electronic resource]
260 $c2014
300 $a13 p.$cil., color.
520 $aMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya.
650 $aCarica papaya
650 $aDisease
650 $aMeleira
650 $aMiRNAs
650 $aPMeV
650 $aVirus
653 $aCarica papaya
653 $aDoenças
653 $aMamão
653 $aMeleira
653 $aMiRNAs
653 $aPMeV
653 $aVírus
700 1 $aGASPAR, C. G.
700 1 $aBUSS, D. S.
700 1 $aVENTURA, J. A.
700 1 $aFERREIRA, P. C. G.
700 1 $aFERNANDES, P. M. B.
773 $tPLOS ONE$gv. 9, issue 7, p. 1-13, july 2014.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Status |
Fechar
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
18/01/2017 |
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Data da última atualização: |
18/01/2017 |
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Tipo da produção científica: |
Publicação em Anais de Congresso |
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Autoria: |
ZUCOLOTO, M.; SANTOS, J. G. dos.; BREGONCI, I. dos S.; ALMEIDA, G. D. de.; VICENTINI, V. B.; MORAES, W. B.; COELHO, R. I. |
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Afiliação: |
Moises Zucoloto, UFES; Juliano Gonçalves dos Santos, UFES; Izaias dos Santos Bregonci, Incaper; Gustavo Dias de Almeida, UFES; Victor Bermardo Vicentini, UFES; Willian Bucker Moraes, UFES; Ruimário Inácio Coelho, UFES. |
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Título: |
Estimativa de área foliar de goiaba (Psidium guajava L.) por meio de dimensões lineares do limbo foliar. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: ENCONTRO LATINO AMERICANO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO LATINO AMERICANO PÓS-GRADUAÇÃO, 7., 2006, São José dos Campos, SP. |
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Idioma: |
Português |
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Conteúdo: |
Objetivando-se obter uma equação que, através de parâmetros lineares dimensionais das folhas, permita estimar a área foliar de P. guajava, estudou-se as relações entre a área foliar real (AF) e parâmetros dimensionais do limbo foliar, como o comprimento ao longo da nervura principal (C) e a largura máxima (L), perpendicular à nervura principal. Os resultados mostram que as equações lineares simples podem ser utilizadas para a estimação da área foliar da goiabeira. Do ponto de vista prático, deve-se optar pela equação linear que envolve o produto C x L, usando-se a equação de regressão AF = 1.2312 + 0.7829CXL, que apresentou resultados mais acurados. |
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Palavras-Chave: |
Área foliar; Estimativa; Goiabeira; Psidium guajava L. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/2626/1/BRT-estimativadaareafoliardagoiaba-bregonci.pdf
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Marc: |
LEADER 01474nam a2200229 a 4500
001 1014082
005 2017-01-18
008 2006 bl uuuu u01u1 u #d
100 1 $aZUCOLOTO, M.
245 $aEstimativa de área foliar de goiaba (Psidium guajava L.) por meio de dimensões lineares do limbo foliar.$h[electronic resource]
260 $aIn: ENCONTRO LATINO AMERICANO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO LATINO AMERICANO PÓS-GRADUAÇÃO, 7., 2006, São José dos Campos, SP.$c2006
520 $aObjetivando-se obter uma equação que, através de parâmetros lineares dimensionais das folhas, permita estimar a área foliar de P. guajava, estudou-se as relações entre a área foliar real (AF) e parâmetros dimensionais do limbo foliar, como o comprimento ao longo da nervura principal (C) e a largura máxima (L), perpendicular à nervura principal. Os resultados mostram que as equações lineares simples podem ser utilizadas para a estimação da área foliar da goiabeira. Do ponto de vista prático, deve-se optar pela equação linear que envolve o produto C x L, usando-se a equação de regressão AF = 1.2312 + 0.7829CXL, que apresentou resultados mais acurados.
653 $aÁrea foliar
653 $aEstimativa
653 $aGoiabeira
653 $aPsidium guajava L
700 1 $aSANTOS, J. G. dos.
700 1 $aBREGONCI, I. dos S.
700 1 $aALMEIDA, G. D. de.
700 1 $aVICENTINI, V. B.
700 1 $aMORAES, W. B.
700 1 $aCOELHO, R. I.
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Biblioteca Rui Tendinha (BRT) |
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